#9800 Anisakidae IgG ELISA
ELISA kit for the diagnosis of digestive anisakidosis in humans
Parasitology and clinical relevance
is caused by some sea fish nematodes of the family Anisakidae. Humans can be infected by eating raw or undercooked
contaminated fish meat. Most infected people do not show any symptoms. However, in
some cases, symptoms appear at the gastric stage (epigastric
pain, nausea and vomiting), intestinal stage (abdominal pain, nausea, vomiting,
and diarrhea) or at the allergic stage (hives, pruritus, angioedema and
bronchospasm). Diagnosis is based on signs and symptoms, but,
in many cases it is not conclusive for the diagnosis. Serological assays
has an important place on the final diagnosis of the disease. Our ELISA kit have
shown appropriate performances for the serological screening of both digestive
and allergic anisakidosis.
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with E/S antigens from cultivated larvae of Anisakidae.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: CH-202201-0009
Sensitivity and specificity
The test detects IgG antibodies
against parasites of Anisakidae family in human serum.
A sensitivity of 97%
was found with 38 sera from patients suffering from digestive and/or
A specificity of 97% was
found with 180 sera of blood donors (Swiss).
of 96% was
found with 98 sera from patients of an infectiology unit (Swiss).
A specificity of
found with 43 sera from anisakidosis suspected patients but where this
disease had been certainly ruled out.
A specificity of 81%
was found with 47 sera of patients with other parasitic infections.
96 wells coated with E/S antigens from
cultivated larvae of Anisakidae.
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 Ál
Cut-off serum (rabbit), 200 Ál
Positive control serum (rabbit), 200 Ál
Protein A - AP conjugate, 300 Ál
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
#9800 Anisakidae IgG ELISA (96 assays)
Raia, D.D., Marotta, C., Costa, A., Ferrantelli, V., Vitale, F. and
(2018) Validation of an Anisakis
in different population groups and public health impact: A systematic
review. Plos one 13.
M., Gonzalez-Munoz, M., Gomez-Morales, M.A., Gottstein, B., Dabrowska, J.,
Rozycki, M., Cencek, T., Muller, N. and Boubaker, G. (2019)
analysis of excretory-secretory antigens of Anisakis simplex,
Pseudoterranova decipiens and Contracaecum osculatum regarding their
applicability for specific serodiagnosis of human anisakidosis based on IgG
Parasitology 197, 9-15.
directive 98/79/EC registration
Material Safety Data Sheet: Eng
- Fra - Deu
Evaluation report (H˘pital Cochin, APHP,
Paris, France) : Report (confidential).
Bordier Affinity Products SA,
Chatanerie 2, BÔtiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
633 31 78. Email: cb(at)bordier.ch