#9750 Trichinella spiralis
ELISA kit for the diagnosis of trichinellosis in humans
Parasitology and clinical relevance
is caused by Trichinella spiralis, a parasitic nematode of pork, horse
and wild animals. Humans can be infected by eating raw or undercooked meat from
infected animals. Most infected people do not show any symptoms. However, in
some cases, symptoms appear during intestinal stage (diarrhea and abdominal
pain<9 and muscular stage (muscle pain, fever, edema). Diagnosis is based
signs and symptoms, but,
in many cases it is not conclusive for the diagnosis. Serological assays
has an important place on the final diagnosis of the disease. Our ELISA kit have
shown appropriate performances for the serological screening of trichinellosis.
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with E/S antigens from cultivated larvae of Trichinella
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: CH-202002-0011
Sensitivity and specificity
The test detects Trichinella
A sensitivity of 95%
was found with 55 sera from patients suffering from trichinellosis.
A specificity of 98% was
found with 149 sera of blood donors (Swiss).
of 98% was
found with 100 sera from patients of an infectiology unit (Swiss).
A specificity of 90%
was found with 62 sera from
trichinellosis suspected patients, but where this disease has been certainly
A specificity of 93%
was found with 45 sera of patients with other parasitic infections.
96 wells coated with E/S antigens from
cultivated larvae of Trichinella spiralis.
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 Ál
Cut-off serum (rabbit), 200 Ál
Positive control serum (rabbit), 200 Ál
Protein A - AP conjugate, 300 Ál
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
#9750 Trichinella spiralis IgG ELISA (96 assays)
M.A., Ludovisi, A., Amati, M., Cherchi, S., Pezzotti, P. and Pozio, E.
Validation of an Enzyme-Linked Immunosorbent Assay for diagnosis of human
trichinellosis. Clin. Vaccine. Immunol. 15: 1723-1729.
Yang, Ya Nan Cai, Ming Wei Tong, Na Sun, Yin Hua Xuan, Yan Jun Kang,
VallÚe, I., Boireau, P., Shi Peng Cheng and Ming Yuan Liu.
Serological tools for detection of Trichinella infection in animals and
humans. One Health 2:
directive 98/79/EC registration
Material Safety Data Sheet: Eng
- Fra - Deu
Evaluation report (H˘pital Cochin, APHP,
Paris, France) : Report
Bordier Affinity Products SA,
Chatanerie 2, BÔtiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
633 31 78. Email: cb(at)bordier.ch