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#9250 Ascaris IgG ELISA
ELISA kit for the diagnosis of ascariasis in humans

Parasitology and clinical relevance

Ascariasis is caused by some soil transmitted helminths of the genus Ascaris. Humans can be infected by ingesting infectious eggs upon contaminated soil, water or food. Most infected people do not show any symptoms. However, in some cases, symptoms appear at the lung stage (persistent cough, shortness of breath and wheezing) or at the intestinal stage (abdominal pain, nausea, vomiting, and diarrhea). Diagnosis is based on the detection of eggs in stool, but, in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease.

Test-Kit

96 determinations of IgG antibodies using standard methodologies.

Wells sensitized with Ascaris coelomic soluble antigens.

8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.

CE registration: CH-202205-0023

Sensitivity and specificity

The test detects IgG antibodies against parasites of Ascaris genus in human serum.

A sensitivity of 81% was found with 27 sera positive with A. suum crude extract antigen and negative with Toxocara canis Bordier ELISA kit.

A specificity of 96% was found with 181 sera of blood donors (Swiss).

A specificity of 98% was found with 96 sera from patients of an infectiology unit (Swiss).

A specificity of 75% was found with 44 sera positive with A. suum crude extract antigen and positive with Toxocara canis Bordier ELISA kit.

A specificity of 57% was found with 65 sera of patients with other parasitic infections.

Contents

96 wells coated with Ascaris coelomic soluble antigens.

Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use

Ordering information

#9250 Ascaris IgG ELISA (96 assays)

References

Schneider, R. and Auer, H. (2016) Incidence of Ascaris suum-specific antibodies in Austian patients with suspected larva migrans visceralis (VLM) syndrome. Parasitology research 115. 

Dana, D., Vlaminck, J., Tadege, B., Mekonnen, Z., Geldhof, P. et al. (2020) Evaluation of copromicroscopy and serology to measure the exposure to Ascaris infections across age groups and to assess the impact of 3 years of biannual mass drug administration in Jimma Town, Ethiopia. Plos Negl Trop Dis 14.

European directive 98/79/EC registration

Material Safety Data Sheet: Eng - Fra - Deu

Bordier Affinity Products SA, Chatanerie 2, Bātiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67.  Fax: + 41 21 633 31 78. Email: cb(at)bordier.ch