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#9700 Taenia solium IgG
ELISA
ELISA kit for the diagnosis of human cysticercosis
Parasitology and clinical relevance
Cysticercosis
is caused by larval cysts of the tapeworm Taenia solium and is a major
cause of adult onset seizures in most developing countries. Diagnosis is based
on imaging findings, but
in many cases it is not conclusive for the diagnosis. Serological assays
has an important place on the final diagnosis of the disease. Our ELISA kit have
shown appropriate performances for the serological screening of cysticercosis.
Test-Kit
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with soluble antigens from cysts of Taenia solium.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: CH-201808-0008 |
Sensitivity and specificity
The test detects Taenia solium
infections.
A sensitivity of 98%
was found with 45 sera from patients suffering from subarachnoid
neurocysticercosis.
A sensitivity of 71%
was found with 45 sera from patients suffering from neurocysticercosis
with only one viable cyst.
A sensitivity of 40%
was found with 45 sera from patients suffering from neurocysticercosis
with only only calcified cyst.
A specificity of 98%
was found with 99 sera of blood donors (Swiss).
A specificity
of 96 % was
found with 100 sera from patients of an infectiology unit (Swiss).
A specificity of 13%
was found with 45 patients suffering
from cystic echinococcosis.A specificity of
71%
was foundwith 45 patients suffering from
hymenolepiasis. |
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Contents
96 wells coated with soluble antigens from
cysts of Taenia solium.
Dilution
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
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Ordering information
#9700 Taenia solium IgG ELISA (96 assays)
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References
J.-F.
Carod, M.Randrianarison, J. Razafimahefa, R.M. Ramahefarisoa, M.
Rakotondrazaka, M. Debruyne, M. Dautigny, P. Cazal, M.L. Andriantseheno,
E.M. Charles. (2011)
Evaluation of the performance of 5 commercialized enzyme immunoassays for
the detection of Taenia solium antibodies and for diagnosis of
neurocysticercosis. Diagnostic Microbiology and Infectious Disease. 72: 85-89.
S.R.V.
Atluri, P. Singhi, N. Khandelwal, N. Malla, (2009)
Neurocysticercosis immunodiagnosis using Taenia solium cysticerci crude
soluble extract, excretory secretory and lower molecular mass antigens in
serum and urine samples of Indian children. Acta Tropica. 110:
22-27.
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European
directive 98/79/EC registration
Material Safety Data Sheet: Eng
- Fra - Deu
Testing of the Bordier Taenia solium
ELISA, final report (Universidad Peruana Cayetano Heredia, Lima, Peru) : Report
Bordier Affinity Products SA,
Chatanerie 2, Bâtiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
633 31 78. Email: cb(at)bordier.ch
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