#9650 Fasciola hepatica
ELISA kit for the diagnosis of fascioliasis in humans
Parasitology and clinical relevance
Fasciolosis is one of the most frequently
encountered autochthonous helminthic infection in Central Europe. The
immunodiagnosis of Fasciola hepatica infections is challenged by high
serological cross-reactivity with other (hepatic) parasitological infections encountered in Central Europe, such as alveolar echinococcosis, toxocarosis and
ascariosis, but also other parasitic infections acquired during overseas travel.
The SAP-2 recombinant antigen shows a high specificity, especially with
patients with other parasitic infections. It is a suitable serological test for routine diagnosis of human fasciolosis, particularly if
are supported by clinical history.
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with a recombinant antigen (SAP2) from Fasciola
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: CH-201504-0006
Sensitivity and specificity
The test detects Fasciola
A sensitivity of 77%
was found with 13 sera from patients with parasitologically proven Fasciola
A specificity of 99%
was found with 99 sera of blood donors (Swiss).
of 98 % was
found with 100 sera from patients of an infectiology unit (Swiss).
A specificity of 97%
was found with patients with other parasitc infections.
96 wells coated with a recombinant
antigen (SAP2) from Fasciola hepatica.
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
complete kit (96 assays) No. 9650
O., Delgado, B. and Espino, A.M.
(2011) Fasciola hepatica saposin-like protein-2-based ELISA for the
serodiagnosis of chronic human fascioliais. Diagnostic Microbiology and
Infectious Disease 70, 355-361.
Gottstein B, Schneeberger M,
Boubaker G, Merkle B, Huber C, et al.
(2014) Comparative Assessment of ELISAs Using Recombinant Saposin-Like
Protein 2 and recombinant Cathepsin L-1 from Fasciola hepatica for the
Serodiagnosis of Human Fasciolosis. PLoS Negl Trop Dis 8(6): e2860.
directive 98/79/EC registration
Material Safety Data Sheet: Eng
- Fra - Deu
Bordier Affinity Products SA,
Chatanerie 2, Bātiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
633 31 78. Email: cb(at)bordier.ch