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#9600 Schistosoma mansoni
IgG ELISA
ELISA kit for the diagnosis of human schistosomiasis
Parasitology and clinical relevance
Two hundred million people are suffering
from schistosomiasis in sub-topical areas. Patients become infected when bathing
in contaminated fresh water. The disease is confirmed by microscopy when eggs are detected in stool or urine
samples. However when the worm burden is light or during the invasive phase, few
or no eggs are excreted. In such situations the serodiagnosis is a useful
complement to parasitolgical tests. The diagnosis of human schistosomiasis using the ELISA kit is intended for
travelers with frequent contacts with fresh water returning from endemic areas,
for travel companions of confirmed cases, to post therapeutic controls and to
control programs in endemic areas.
Test-Kit
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with soluble antigens from Schistosoma mansoni.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: H-CH/CA01/IVD/17983 |
Sensitivity and specificity
The test detects both Schistosoma
mansoni and Schistosoma haematobium infections.
A sensitivity of 94%
was found with sera from patients with parasitologically proven Schistosoma
mansoni and/or Schistosoma haematobium infections.
A specificity of 99%
was found with 122 sera of blood donors.
A specificity of 94%
was found with patients with other parasitc infections. |
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Contents
96 wells coated with soluble antigens from
Schistosoma mansoni.
Dilution
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
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Ordering information
#9600 Schistosoma mansoni IgG ELISA (96 assays)
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References
Doenhoff, M.J., Wheeler, J.G.,
Tricker, K., Hamilton, J.V., Sturrock, R.F., Butterworth, A.E., Ouma, J.H.,
Mbugua, G.G., Kariuki, C. and Koech, D. (2003) The detection of
antibodies against Schistosoma mansoni soluble egg antigens (SEA) and
CEF6 in ELISA, before and after chemotherapy. Ann. Trop. Med. Parasitol. 97,
697-709.
Turner, P., Lalloo, K., Bligh, J.,
Amstrong, M., Whitty, C.J.M., Doenhoff, M.J. and Chiodini, P.L. (2004)
Serological speciation of human schistosome infections by ELISA with a
panel of three antigens. J. Clin. Pathol. 57, 1193-1196.
Sorgho, H., Bahgat, M., Poda, J., Song, W., Kristen,
C., Doenhoff, M.J., Zongo, I., Ouedraogo, J. and Ruppel, A. (2005)
Serodiagnosis of Schistosoma mansoni infections in endemic area of
Burkina Faso: performance of several immunological tests with different
parasite antigens. Acta
Tropica. 93, 169-180. |
European
directive 98/79/EC registration
Material Safety Data Sheet: Eng
- Fra - Deu
Evaluation of a novel ELISA for
Schistosomiasis serology: Poster
Bordier Affinity Products SA,
Chatanerie 2, Bâtiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
633 31 78. Email: cb(at)bordier.ch
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