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#9600 Schistosoma mansoni
ELISA kit for the diagnosis of schistosomiasis in humans

Parasitology and clinical relevance

Two hundred million people are suffering from schistosomiasis in sub-topical areas. Patients become infected when bathing in contaminated fresh water. The disease is confirmed by microscopy when eggs are detected in stool or urine samples. However when the worm burden is light or during the invasive phase, few or no eggs are excreted. In such situations the serodiagnosis is a useful complement to parasitolgical tests. The diagnosis of human schistosomiasis using the ELISA kit is intended for travelers with frequent contacts with fresh water returning from endemic areas, for travel companions of confirmed cases, to post therapeutic controls and to control programs in endemic areas.

Test-Kit

96 determinations of IgG antibodies using standard methodologies.

Wells sensitized with soluble antigens from Schistosoma mansoni.

8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.

CE registration: H-CH/CA01/IVD/17983

Sensitivity and specificity

The test detects both Schistosoma mansoni and Schistosoma haematobium infections.

A sensitivity of 94% was found with sera from patients with parasitologically proven Schistosoma mansoni and/or Schistosoma haematobium infections.

A specificity of 99% was found with 122 sera of blood donors.

A specificity of 94% was found with patients with other parasitc infections.

Contents

96 wells coated with soluble antigens from Schistosoma mansoni.

Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 l
Cut-off serum (rabbit), 200 l
Positive control serum (rabbit), 200 l
Protein A - AP conjugate, 300 l
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use

Ordering information

Schistosoma mansoni
complete kit (96 assays) No. 9600

References

Doenhoff, M.J., Wheeler, J.G., Tricker, K., Hamilton, J.V., Sturrock, R.F., Butterworth, A.E., Ouma, J.H., Mbugua, G.G., Kariuki, C. and Koech, D. (2003) The detection of antibodies against Schistosoma mansoni soluble egg antigens (SEA) and CEF6 in ELISA, before and after chemotherapy. Ann. Trop. Med. Parasitol. 97, 697-709.

Turner, P., Lalloo, K., Bligh, J., Amstrong, M., Whitty, C.J.M., Doenhoff, M.J. and Chiodini, P.L. (2004) Serological speciation of human schistosome infections by ELISA with a panel of three antigens. J. Clin. Pathol. 57, 1193-1196.

Sorgho, H., Bahgat, M., Poda, J., Song, W., Kristen, C., Doenhoff, M.J., Zongo, I., Ouedraogo, J. and Ruppel, A. (2005) Serodiagnosis of Schistosoma mansoni infections in endemic area of Burkina Faso: performance of several immunological tests with different parasite antigens. Acta Tropica. 93, 169-180.

European directive 98/79/EC registration

Material Safety Data Sheet: Eng - Fra - Deu

Evaluation of a novel ELISA for Schistosomiasis serology: Poster

 

Bordier Affinity Products SA, Chatanerie 2, Btiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67.  Fax: + 41 21 633 31 78. Email: cb(at)bordier.ch

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