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#9550 Entamoeba histolytica
IgG ELISA
ELISA kit for the diagnosis of human visceral amebiasis
Parasitology and clinical relevance
Fifty million people are
suffering from extra-intestinal amebiasis in the world. It is responsible of fifty
thousand death each year. It is the second cause of mortality by parasitic
infections, after Malaria. Patients become infected when eating contaminated
vegetables or water. The disease is confirmed by microscopy when the hematophagous form of Entamoeba
histolytica
are detected in stool samples. However this vegetative form are frequently
absent or present in very small numbers. Therefore, a serological assay to
detect infection of E. histolytica is a useful complement to
parasitological tests. The diagnosis of human amebiasis using the ELISA kit is intended for travellers
returning from endemic areas and developing symptoms like abdominal pain, fever
and hepatomegaly and to post therapeutic controls.
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Test-Kit
96
determinations of IgG antibodies using standard methodologies.
Wells sensitized with soluble trophozoite antigens from Entamoeba
histolytica.
8-wells polystyrene breakable strips: useful and economic format for
laboratories with a small workload.
CE registration:
CH-201202-0033 |
Sensitivity
and specificity
The test detects Entamoeba
histolytica extra-intestinal infections.
A sensitivity of 100%
was found with 52 sera from patients with clinically proven Entamoeba
histolytica extra-intestinal infection.
A specificity of 89% was
found with 71 sera from amebiasis suspected patients but where this
disease had been certainly ruled out.
A specificity of 96% was found with 99 sera of blood donors.
A
specificity of 80% was found with 40 sera from patients
with other parasitc infections. |
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Contents
96 wells coated with
soluble trophozoite
antigens from Entamoeba histolytica.
Dilution
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
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Ordering information
#9550
Entamoeba
histolytica IgG ELISA (96 assays)
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References
Nicholls, R.S.,
I Restrepo, M., Duque, S., Consuelo Lopez, M. and Corredor A. (1994)
Standardization and evaluation of ELISA for the serodiagnostic of amoebic
liver abscess. Mem. Inst. Oswaldo Cruz, Rio de Janeiro. 89, 53-58.
Visser, L.G., Verweij,
J.J., Van Esbroeck, M., Edeling, W.M., Clerinx, J. and Polderman, A.M. (2006)
Diagnostic methods for differentiation of E. histolytica and E.
dispar in carriers : performance and clinical implications in a
non-endemic setting. Int. J. Med. Microbiol. 296,
397-403.
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European
directive 98/79/EC registration
Material Safety Data Sheet: Eng
- Fra - Deu
Flyer Entamoeba histolytica IgG ELISA: Flyer
Bordier Affinity Products SA,
Chatanerie 2, Bātiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
633 31 78. Email: cb(at)bordier.ch
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