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#9500 Leishmania
infantum IgG ELISA
ELISA kit for the diagnosis of human and canine visceral leishmaniasis
Parasitology and clinical relevance
The parasites of the genus Leishmania are
intracellular protists transmitted by an insect vector. Traditionally three forms of
leishmaniases are clinically distinguished : One of these, visceral leishmaniasis, is
caused by Leishmania infantum in the Mediterranean area and in South America and
by Leishmania donovani in Eastern Africa and in Southern Asia. This infection affects
millions of individuals and is frequently lethal if left untreated. Recently a growing
number of patients developed visceral leishmaniasis during a co-infection with HIV.
Epidemiologically, the domestic dog is very sensitive to infection by L. infantum
and is an important reservoir host for the transmission of the human disease. The culture
of the parasite from biopsies or a direct detection of amastigotes in tissue smears are
definitve proof for the presence of the parasite. However, as the antibody titers are
usually elevated in the absence of HIV co-infection, serology is conveniently used as a
less invasive procedure for the diagnosis and the follow-up during treatment of visceral
leishmaniasis.
Test-Kit
96 determinations of IgG antibodies using standard
methodologies.
Wells sensitized with soluble antigens from cultured Leishmania
infantum promastigotes.
8-wells polystyrene breakable strips : useful and
economic format for laboratories with a small workload.
CE registration: H-CH/CA01/IVD/01756 |
Sensitivity
and specificity
Sensitivity with immunocompetent patients
>95% and
Leishmania-HIV co-infections : 60-85%
Specificity (Swiss
blood donors) >95%
Cross-reactivity occurs with
trypanosomiasis and to
some extent with leprosy and tuberculosis. The
conjugate used in this kit (protein A) allows the detection of antibodies
in the serum of humans and dogs. |
Contents
96 wells coated with Leishmania infantum promastigote soluble
antigens.
Dilution
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (canine), 200 µl
Cut-off serum (canine), 200 µl
Positive control serum (canine), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
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Ordering
information
#9500
Leishmania infantum IgG ELISA (96 assays)
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References
Badaro,
R., Reed, S.G., Barral, A., Orge, G., Jones, T.C.
(1986) Evaluation of the micro enzyme-linked immunosorbent assay (ELISA)
for antibodies in american visceral leishmaniasis : antigen selection
for detection of infection specific responses. Am. J. Trop. Med. Hyg. 35: 72-78.
Daleine, G., Deniau, M., Matheron, S., Leport, C., Lebras, J. (1994)
Leishmaniose viscérale au cours de l’infection à VIH : avantages
du sérodiagnotic par technique ELISA. Pres. Med. 23 : 672-673.
Nassar, N., Gangneux, J.P., Sulahian, A., Derouin, F.
(1996) Leishmaniose viscérale au cours du sida : Difficultés du
diagnostic sérologique, évaluation d’une nouvelle trousse ELISA. Feuillets
de Biologie. 37: 39-42.
Senaldi,
G., Xiao-su, H., Hoessli, D.C., Bordier, C.
(1996) Serological diagnosis of visceral leishmaniasis by a dot-enzyme
immunoassay for the detection of a Leishmania
donovani-related circulating antigen. Journal of Immunological
Methods. 193: 9-15.
Maia,
C., Nunes, M., Cristovao, J., Campino, L.
(2010) Experimental canine leishmaniasis: Clinical, parasitological and
serological follow-up. Acta Tropica. 116: 193-199.
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European directive 98/79/EC registration
Material Safety Data Sheet: Eng
- Fra - Deu
Clinical evaluations of the kit (french presentations):
Delaunay, P. et al., Société Française de Parasitologie, Paris 2002: Diaporama
Eiselé, L. et al.,
Société Française de Parasitologie, Paris 2002: Diaporama
Gharbi, M. et al., Société Française de Parasitologie, Marseille 2003: Poster
Bordier Affinity Products SA,
Chatanerie 2, Bâtiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
633 31 78. Email: cb(at)bordier.ch
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