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# 9450 Strongyloïdes ratti
IgG ELISA
ELISA kit for the diagnosis of human strongyloidiasis
Parasitology and clinical relevance
Strongyloides stercoralis is a potentially dangerous parasite, particularly if unsuspected and present in immunocompromised patients or in patients receiving immunosuppressive drugs. An hyperinfection syndrome can occur with widespread nematode dissemination and a high mortality.
Parasitological diagnosis is often time- consuming as Strongyloides larvae in the stool are frequently absent or present in very small numbers. Therefore, a
serological assay to detect infection with Strongyloides is useful. Our ELISA uses the
structural similarities existing between
S. stercoralis and S. ratti antigens to detect antibodies in human sera.
Test-Kit
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with somatic antigens from larvae of Strongyloïdes
ratti.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: H-CH/CA01/IVD/10285 |
Sensitivity and specificity
Sensitivity of 88% with sera from patients with larvae
of Strongyloides stercoralis.
Specificity of 94% with sera of blood donors (from
Switzerland).
Specificity of 77% with sera of patients with other parasitoses
(mostly helminthiases). |
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Contents
96 wells coated with somatic antigens from
larvae of Strongyloïdes ratti.
Dilution
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
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Ordering information
#9450 Strongyloïdes ratti IgG ELISA (96 assays)
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References
Magnaval, J.-F., Mansuy, J.M., Villeneuve, L. and Cassaing, S. (2000) A
retrospective study of autochthonous strongyloïdiasis in Région
Midi-Pyrénées (Southwesten France). Eur. J. Epidemiol. 16, 179-182.
Schaffel, R., Nucci, M., Carvalho, E., Braga, M., Almeida. L., Portugal, R.
and Pulcheri, W. (2001) The value of an immunoenzymatic test (ELISA)
for the diagnosis of Strongyloïdiasis in patients immunosuppressed by
hematologic malignancies. Am. J. Trop. Med. Hyg. 65, 346-350.
Loutfy, M.R., Wilson, M., Keystone, J.S. and Kain, K.C. (2002) Serology
and eosinophil count in the diagnosis and management of strongyloïdiasis in
non endemic area. Am. J. Trop. Med. Hyg. 66, 749-752. Van
Doorn, H.R., Koelewijn, R., Hofwegen, H., Gilis, H., Wetsteyn, J.C.F.M.,
Wismans, P.J., Sarfati, C., Vervoort, T. and Gool, T. (2007) Use of
ELISA and dipstick assay for the detection of Strongyloïdes stercoralis
infection in humans. J. Clin. Microbiol. 45, 438-442. Bisoffi,
Z., Buonfrate, D., Sequi, M., Mejia, R., Cimino, R.O. et al.
(2014) Diagnostic accuracy of five serologic tests for Strongyloïdes
stercoralis infection. Plos. Negl. Trop. Dis. 8.
Buonfrate,
D., Sequi, M., Mejia, R., Cimino, R.O., Kroleiecki, A., Albonico, M., et
al. (2015)
Accuracy of Five Serologic Tests for the follow-up of Strongyloïdes
stercoralis infection. PLos Negl. Trop. Dis. 9.
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European
directive 98/79/EC registration
Material Safety Data Sheet: Eng
- Fra - Deu
Assessment of a new ELISA test for the serodiagnosis of
Strongyloïdiasis: Poster presentation
Assessment of a new ELISA test for the serodiagnosis of
Strongyloïdiasis: French poster presentation
Evaluation of the Bordier Strongyloïdes
ratti ELISA kit for the diagnosis of human Strongyloïdiasis (Center
for Disease Control, Atlanta, USA): Report
Prevalence and risk factors for Strongyloïdes
stercoralis infection among patients at high risk of complications in
Bolivia: Poster presentation
Bordier Affinity Products SA, Chatanerie 2,
Bâtiment Biokema,
CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
633
31 78. Email: cb(at)bordier.ch
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