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 #9400 Acanthocheilonema viteae
ELISA kit for the diagnosis of filariasis in humans

Parasitology and clinical relevance

Human filariasis are caused by a variety of nematodes (Loa Loa, Onchocerca volvulus, Dracunculus medinensis, Wuchereria bancrofti, Brugia malayi). The diagnosis is based on clinical evidences, an history of living in an endemic area, hypereosinophilia and the detection of microfilariae. The finding of an adult filaria is a proof of infection but is frequently unsuccessful. Serological methods using indirect immunofluorescence assays on frozen sections of adult worms of animal origin (Acanthocheilonema viteae, Dirofilaria immitis, Setaria sp.) and immuno-electrophoresis can both help for diagnosis. These methods are often positive in cases of occult filariasis or when microfilaremia is negative, they are less sensitive in patients with detectable microfilariae. Our ELISA assay uses also the antigenic homologies observed between human and animal filariae. The ELISA procedure is more reproducible and less tedious than IFA on worm sections.

Test-Kit

96 determinations of IgG antibodies using standard methodologies.


Wells sensitized with somatic antigens from adult females of Acanthocheilonema viteae.


8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.

CE registration: H-CH/CA01/IVD/01755

Sensitivity and specificity

Sensitivity of 95% with sera of suspected and confirmed cases of filariasis.

Specificity of 98% with sera of blood donors (from Switzerland).

Crossreactivities are found in cases of trichinellosis, hydatidosis, fascioliasis, ankylostomiasis and strongyloidiasis (40 to 83%).

Contents

96 wells coated with somatic antigens from adult females of Acanthocheilonema viteae.

Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use

Ordering information

Acanthocheilonema viteae
complete kit (96 assays) No. 9400

References

Gueglio, B., Bordier, C. et Marjollet, M. (1995) Mise au point d'un test ELISA pour le diagnostic des filarioses humaines. Bulletin de la Société Française de Parasitologie. 13, 67-72.

Laverbratt, C., Ljungström, I., Guzman, G., Thors, C., Eriksson, T. and Akuffo, H.O. (1997) Evaluation of serological assays for diagnosis of onchocercosis. Scand. J. Infect. Dis. 29, 65-70.

European directive 98/79/EC registration

Material Safety Data Sheet:  Eng - Fra - Deu

Assessment of a new ELISA test for the serodiagnosis of Filariasis: French poster presentation

 

Bordier Affinity Products SA, Chatanerie 2, Bâtiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67.  Fax: + 41 21 633 31 78. Email: cb(at)bordier.ch

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