#9350 Echinococcus granulosus
ELISA kit for the diagnosis of echinococcoses in
Parasitology and clinical
Cystic hydatid disease is caused by an infection with the cestode Echinococcus
granulosus and is potentially dangerous for humans. New cases are mostly found in
areas where dogs and livestock coexist. Adult worms mature in the intestine of the dog
(definitive host) and the eggs are released in the feces. By an accidental ingestion in
humans, oncospheres hatch in the duodenum, penetrate the intestine and are carrieds via
the bloodstream to various organs. Hydatid cysts form in organs like liver (60-70 %),
lungs and brain. The metacestode cyst develops over years in the patient and can, in most
cases, be surgically removed after diagnosis. Medical imaging and serology give both
usefull and complementary information on the nature of the cyst and concerning the issue
of surgical intervention. The test uses an antigen preparation (hydatid fluid) which
detects indiscriminately antibodies from patients with cystic hydatid disease (E.
granulosus) or with alveolar echinococcosis (E. multilocularis).
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with antigens from Echinococcus granulosus hydatid
8-wells polystyrene breakable strips : useful and economic format for laboratories
with a small workload.
CE registration: H-CH/CA01/IVD/01757
Sensitivity and specificity
96% on cystic hydatid diseases (E. granulosus).
90% on alveolar echinococcoses (E. multilocularis).
Specificity : 97% negativity on blood donors (from
18% with helminthiases.
17% with protozooses.
In case of positive results, the use of the Em2-Em18 ELISA (No 9300) and a search for
anti-8 kD band antibodies by western blot are recommended for a species-specific diagnosis.
96 wells coated with Echinococcus granulosus antigens (hydatid fluid).
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
complete kit (96 assays) No. 9350
Gottstein, B. (1992) Molecular and immunological diagnosis of
Echinococcosis. Clin. Microbiol. Rev. 5, 248-261.
Jacquier, P., Bresson-Hadni, S. and Eckert, J. (1993) Improved primary
immunodiagnosis of alveolar Echinococcosis in humans by an ELISA using the
Em2plus antigen. J. Clin. Microbiol. 31, 373-376.
D., Felleisen, E., Grimm, F., Pfister, M., Teuscher, F., Zuercher, C.,
Reichen, J. and Gottstein, B. (1999) Differential immunodiagnosis
between cystic hydatid disease and other cross-reactive pathologies. Am.
J. Trop. Med. Hyg. 60, 193-198.
Material Safety Data Sheet (MSDS) : Eng
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Bordier Affinity Products SA, Chatanerie 2,
CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
31 78. cb (at) bordier.ch