#9300 Echinococcus multilocularis
ELISA kit for the diagnosis of alveolar echinococcosis in
Parasitology and clinical relevance
Alveolar echinococcosis is caused by an infection with the cestode Echinococcus
multilocularis and is a very dangerous disease for humans. Adult worms mature in the
intestine of the definitive host (usually fox, occasionally dog or cat) and the eggs are
released in the feces. Accidental ingestion of soil, berries or vegetables contaminated
with eggs iniate the infection in humans. Oncospheres hatch in the duodenum, penetrate the
intestine and are carried via the bloodstream to organs. Although alveolar hydatid
cysts have been found in other tissues, the most common site of development is the liver.
The cysts are not limited by a membrane but penetrate the tissues and may resemble a slow
growing carcinoma. Surgical removing is difficult but drugs have been found that stop the
development of the metacestode. Medical imaging and serology give both useful and
complementary information on the nature of the cyst and on the outcome of treatment.
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with affinity purified and recombinant antigens
(Em2-Em18) from Echinococcus multilocularis.
8-well polystyrene breakable strips : useful and economic format for
laboratories with a small workload.
CE registration: H-CH/CA01/IVD/01757
Sensitivity and specificity
Sensitivity of 83% on alveolar echinococcosis (E. multilocularis).
Specificity of 98% (blood donors from Switzerland,
cancer patients, ... ).
~7% with other parasitoses.
~16% with hydatidosis (E. granulosus).
96 wells coated with Echinococcus multilocularis Em2-Em18 antigens.
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
complete kit (96 assays) No. 9300
Müller, N., Gottstein, B.,
Vogel, M., Flury, K. and Seebeck, T. (1989)
Application of a recombinant Echinococcus multilocularis antigen in
an ELISA for immunodiagnosis of human alveolar echinococcosis.
Parasitol. 36 : 151-160.
B., Jacquier, P., Bresson-Hadni, S. and Eckert, J.
(1993) Improved primary immunodiagnosis of alveolar Echinococcosis in
humans by an enzyme-linked immunosorbent assay using the Em2plus
antigen. J. Clin.
Microbiol. 31 : 373-376.
J., Conraths, F. and Tackmann, K. (2000)
Echinococcosis: an emerging or re-emerging zoonosis? Int.
J. Parasitol. 30 :
Müller, N., Frei, E., Nuñez,
S. and Gottstein, B. (2006) Improved
serodiagnosis of alveolar echinococcosis of humans using an in
vitro-produced Echinococcus multilocularis antigen. Parasitology. 134 :
Knapp, J., Sako, Y.,
Grenouillet, F. Bresson-Hadni, S. Richou, C., Gbaguidi-Haore, H., Ito, A.,
Millon, L. (2014) Comparison of the
serological tests ICT and ELISA for the diagnosis of alveolar
echinococcosis in France. Parasite.
Material Safety Data Sheet: Eng
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Bordier Affinity Products SA, Chatanerie 2,
CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
31 78. Email: cb(at)bordier.ch