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#9300 Echinococcus multilocularis (Em2-Em18)
ELISA kit for the diagnosis of alveolar echinococcosis in humans

Parasitology and clinical relevance

Alveolar echinococcosis is caused by an infection with the cestode Echinococcus multilocularis and is a very dangerous disease for humans. Adult worms mature in the intestine of the definitive host (usually fox, occasionally dog or cat) and the eggs are released in the feces. Accidental ingestion of soil, berries or vegetables contaminated with eggs iniate the infection in humans. Oncospheres hatch in the duodenum, penetrate the intestine and are carried via the bloodstream to organs. Although alveolar hydatid cysts have been found in other tissues, the most common site of development is the liver. The cysts are not limited by a membrane but penetrate the tissues and may resemble a slow growing carcinoma. Surgical removing is difficult but drugs have been found that stop the development of the metacestode. Medical imaging and serology give both useful and complementary information on the nature of the cyst and on the outcome of treatment.

Test-Kit

96 determinations of IgG antibodies using standard methodologies.

Wells sensitized with affinity purified and recombinant antigens (Em2-Em18) from Echinococcus multilocularis.

8-well polystyrene breakable strips : useful and economic format for laboratories with a small workload.

CE registration:  H-CH/CA01/IVD/01757

Sensitivity and specificity

Sensitivity of 83% on alveolar echinococcosis (E. multilocularis).

Specificity of 98% (blood donors from Switzerland, cancer patients, ... ).

Cross-reactivities :

~7% with other parasitoses.
~16% with hydatidosis (E. granulosus).

Contents

96 wells coated with Echinococcus multilocularis Em2-Em18 antigens.

Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 l
Cut-off serum (rabbit), 200 l
Positive control serum (rabbit), 200 l
Protein A - AP conjugate, 300 l
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use

Ordering information

Echinococcus multilocularis (Em2-Em18)
complete kit (96 assays) No. 9300

References

Mller, N., Gottstein, B., Vogel, M., Flury, K. and Seebeck, T. (1989) Application of a recombinant Echinococcus multilocularis antigen in an ELISA for immunodiagnosis of human alveolar echinococcosis.  Mol.  Biochem.  Parasitol. 36 : 151-160.

Gottstein, B., Jacquier, P., Bresson-Hadni, S. and Eckert, J. (1993) Improved primary immunodiagnosis of alveolar Echinococcosis in humans by an enzyme-linked immunosorbent assay using the Em2plus antigen.  J. Clin.  Microbiol. 31 : 373-376.

Eckert, J., Conraths, F. and Tackmann, K. (2000) Echinococcosis: an emerging or re-emerging zoonosis? Int. J. Parasitol. 30 : 1283-1294.

Mller, N., Frei, E., Nuez, S. and Gottstein, B. (2006) Improved serodiagnosis of alveolar echinococcosis of humans using an in vitro-produced Echinococcus multilocularis antigen. Parasitology. 134 : 1-10.

Knapp, J., Sako, Y., Grenouillet, F. Bresson-Hadni, S. Richou, C., Gbaguidi-Haore, H., Ito, A., Millon, L. (2014) Comparison of the serological tests ICT and ELISA for the diagnosis of alveolar echinococcosis in France. Parasite. 21.

European directive 98/79/EC registration

Material Safety Data Sheet: Eng - Fra - Deu

 

Bordier Affinity Products SA, Chatanerie 2, Btiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67.  Fax: + 41 21 633 31 78. Email: cb(at)bordier.ch

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