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#9200 Toxocara canis
ELISA kit for the diagnosis of toxocariasis in humans and dogs

Parasitology and clinical relevance

Human toxocariasis (visceral larva migrans) is caused by an infection with the round worm Toxocara ssp. larvae. Dogs infected with adult worms release in their feces a large number of infectious eggs into the environment. After ingestion of eggs by the patient, the infecting larvae begin a somatic migration through the inner organs, releasing immunogenic compounds globally known as excretory/secretory (E/S) antigens. Clinical manifestations of toxocarosis include frequently a marked hypereosinophilia and also asthenia, abdominal pain or asthma. Larvae remain most frequently located in the liver and the lungs but can also cause serious ocular damage by migrating into the retina (ocular larva migrans). As the direct detection of larvae is not feasible in humans, a serological assay, on E/S antigens, is presently the most sensitive method available for the diagnosis of toxocariasis.

Test-Kit

96 determinations of IgG antibodies using standard methodologies.

Wells sensitized with E/S antigens of cultured Toxocara canis larvae.

8-wells polystyrene breakable strips : useful and economic format for laboratories with a small workload.

CE registration:  H-CH/CA01/IVD/01755

Sensitivity and specificity

Sensitivity 91%

S
pecificity 96% with 500 sera of blood donnors (Swiss).Contacts without any clinical symptoms may have titers between the negative and the cut-off serums.

Specificity 86% with regards to other parasitic infections. Crossreactivity mainly occur in patients with Trichinellosis, fascioliasis, amebiasis and strongyloidiasis.

The conjugate used in this kit (protein A) allows the detection of antibodies in the serum of humans and dogs.

Contents

96 wells coated with Toxocara canis E/S antigens.

Dilution buffer (TBS-Tween),  50 ml
Washing solution,  50 ml
Enzyme buffer,  50 ml
Stop solution,  25 ml
Negative control serum (rabbit),  200
ml
Cut-off serum (rabbit),  200
ml
Positive control serum (rabbit),  200
ml
Anti-human IgG AP conjugate,  300
ml
Alk. Phosphatase substrate,  20 tablets
Multipipette reservoir, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use

Ordering information

Toxocara canis
complete kit (96 assays) No. 9200

Toxocara canis
E/S antigens (96 assays) No. 9220

References

De Savigny, D.H., Voller, A. and Woodruff, A.W. (1979) Toxocariasis: serological diagnosis by enzyme immunoassay. J. Clin. Path. 32 : 284-288.

Speiser, F. and Gottstein, B.
(1984) A collaborative study on larval excretory/secretory antigens of Toxocara canis for the immunodiagnosis of human toxocariasis with ELISA. Acta Tropica. 41, 361-372.

Jacquier, P., Gottstein, B., Singelin, Y. and Eckert, J. (1991) Immunodiagnosis of Toxocariasis in Human: Evaluation of a New Enzyme-Linked Immunosorbent Asay Kit. J. Clin. Microbiol. 29, 1831-1835.

Choi, D., Lim, J.H., Choi D-C., Paik, S-W. and Huh, S. (2008) Toxocariasis and Ingestion of Raw Cow Liver in Patients with Eosinophilia. Korean J. Parasitol. 46, 139-143.

Jin, Y., Shen, C., Huh, S., Sohn, W-M., Choi, M-H. and Hong, S-T. (2013) Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae. Korean J. Parasitol. 51, 433-439.

European directive 98/79/EC registration

Material Safety Data Sheet: Eng - Fra - Deu

 

Bordier Affinity Products SA, Chatanerie 2, Bâtiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67.  Fax: + 41 21 633 31 78. Email: cb(at)bordier.ch

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