#9200 Toxocara canis
ELISA kit for the diagnosis of toxocariasis in humans
Parasitology and clinical relevance
Human toxocariasis (visceral larva migrans) is
caused by an infection with the round worm Toxocara ssp. larvae. Dogs infected with adult
worms release in their feces a large number of infectious eggs into the environment. After
ingestion of eggs by the patient, the infecting larvae begin a somatic migration through
the inner organs, releasing immunogenic compounds globally known as excretory/secretory
(E/S) antigens. Clinical manifestations of toxocarosis include frequently a marked
hypereosinophilia and also asthenia, abdominal pain or asthma. Larvae remain most
frequently located in the liver and the lungs but can also cause serious ocular damage by
migrating into the retina (ocular larva migrans). As the direct detection of larvae
is not feasible in humans, a serological assay, on E/S antigens, is presently the most
sensitive method available for the diagnosis of toxocariasis.
96 determinations of IgG antibodies using standard
Wells sensitized with E/S antigens of cultured Toxocara canis
8-wells polystyrene breakable strips : useful and economic format for laboratories
with a small workload.
CE registration: H-CH/CA01/IVD/01755
Sensitivity and specificity
96% with 500 sera of blood donnors (Swiss).Contacts
without any clinical symptoms may have titers between the negative and the cut-off serums.
86% with regards to other parasitic infections. Crossreactivity
mainly occur in patients with Trichinellosis, fascioliasis, amebiasis and
conjugate used in this kit (protein A) allows the detection of antibodies
in the serum of humans and dogs.
96 wells coated with Toxocara canis E/S antigens.
Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 ml
Cut-off serum (rabbit), 200 ml
Positive control serum (rabbit), 200 ml
Anti-human IgG AP conjugate, 300 ml
Alk. Phosphatase substrate, 20 tablets
Multipipette reservoir, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
complete kit (96 assays) No. 9200
E/S antigens (96 assays) No. 9220
Savigny, D.H., Voller, A. and Woodruff, A.W. (1979) Toxocariasis:
serological diagnosis by enzyme immunoassay. J. Clin. Path. 32 :
Speiser, F. and Gottstein, B. (1984) A collaborative study on
larval excretory/secretory antigens of Toxocara canis for the
immunodiagnosis of human toxocariasis with ELISA. Acta Tropica. 41,
Jacquier, P., Gottstein, B., Singelin, Y. and Eckert, J. (1991)
Immunodiagnosis of Toxocariasis in Human: Evaluation of a New
Enzyme-Linked Immunosorbent Asay Kit. J. Clin. Microbiol. 29, 1831-1835.
Choi, D., Lim, J.H., Choi D-C., Paik, S-W. and Huh, S. (2008)
Toxocariasis and Ingestion of Raw Cow Liver in Patients with Eosinophilia.
Korean J. Parasitol. 46, 139-143.
Jin, Y., Shen, C., Huh, S., Sohn, W-M., Choi, M-H. and Hong, S-T. (2013)
Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara
canis Larvae. Korean J. Parasitol. 51, 433-439.
European directive 98/79/EC registration
Material Safety Data Sheet: Eng
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