|
#6100 Aspergillus fumigatus IgG ELISA
ELISA
kit for the diagnosis of human aspergillosis
Parasitology
and clinical relevance
Aspergillus
fumigatus is one of the most ubiquitous of the airborne saprophytic fungi.
Humans and animals constantly inhale numerous conidia of this fungus. It is
responsible from mycosis occuring on patients suffering from lung disorder such
as asthma, cistic fibrosis and COBP. Aspergillosis is the most common cause
among fungal respiratory diseases. This
test is useful for the screening and follow up of patients at risk of aspergillosis. We
associate a highly specific somatic and metabolic antigen with
two recombinant antigens (Chymotrypsin and Mitogillin).The
complementarity of these antigens gives excellent performance to the test.
Test-Kit
96 determinations of IgG antibodies using standard methodologies.
Wells
sensitized with soluble somatic, metabolic and recombinant antigens from
Aspergillus
fumigatus.
8-wells polystyrene breakable strips: useful and economic format for
laboratories with a small workload.
CE
registration: CH-201301-0006
|
Sensitivity and specificity
A
sensitivity of 97% was found with 32 sera from 19 patients
suffering from various aspergillosis (11 chronic necrotizing
aspergillosis, 12 aspergilloma, 2 aspergillus sinusitis and 7 allergic
bronchopulmonary aspergillosis).
A
sensitivity of 22% was found with 9 sera from 5 patients suffering from
invasive d'aspergillosis.
A
specificity of 98% was found with 131 sera from 67 patients
suffering from non aspergillus respiratory diseases (candidosis,
tuberculosis, pneumocystosis, cryptococcosis, viral or bacterial
pneumonia).
|
|
Contents
96
wells coated with soluble somatic, metabolic and recombinant antigens from
Aspergillus fumigatus.
Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
|
Ordering
information
#6100
Aspergillus
fumigatus IgG ELISA (96 assays)
|
|
References
Weig,
M., Frosch, M., Tintelnot, K., Haas, A., Gross. U., Linsmeier, B. and
Heesemann, J. (2001). Use
of recombinant mitogillin for improved serodiagnosis of Aspergillus
fumigatus associated diseases. J. Clin. Microbiol. 39,
1721-1730.
Sarfati,
S., Monod, M., Recco, P., Sulahian, A., Pinel, C., Candolfi, E., Fontaine,
T., Debeaupuis, J.P., Tabouret, M. and Latgé, J.P. (2006). Recombinant
antigens as diagnostic markers for aspergillosis. Diag.
Microbiol. Inf. Disease 55, 279-291.
Dumollard C, Bailly S, Perriot S,
Brenier-Pinchart MP, Saint-Raymond C, Camara B, Gangneux JP, Persat F,
Valot S, Grenouillet F, Pelloux H, Pinel C, Cornet M. Prospective
evaluation of a new Aspergillus IgG EIA kit for the diagnosis of chronic
and allergic pulmonary aspergillosis. J
Clin Microbiol. 2016 Feb 17. pii: JCM.03261-15.
|
European
directive 98/79/EC registration
Material Safety Data Sheet: Eng
- Fra - Deu
New ELISA test for the serodiagnosis of
Aspergillosis : complementarity of the association between recombinant antigens
and somatic/metabolic antigens: French poster
presentation
Prospective evaluation of a new ELISA kit for
the detection of anti-Aspergillus IgG in the diagnosis of chronic and
allergic pulmonary aspergillosis: French
poster presentation & English Flyer
Prospective
evaluation of a new Aspergillus IgG EIA kit for the diagnosis of chronic
and allergic pulmonary aspergillosis: Study
paper
Bordier Affinity Products SA, Chatanerie 2,
Bātiment Biokema,
CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
633
31 78. Email: cb(at)bordier.ch
|